Senegal striker in Bayern Munich’s Bundesliga fixture against Werder Bremen, 13 days before his country are due to play their World Cup opener.
The 30-year-old left the field after 20 minutes – with Bayern leading 2-1 – and headed down the tunnel.
Senegal face the Netherlands on 21 November, one day after the World Cup begins in Qatar.
League leaders Bayern pushed on to a 4-1 half-time lead in Mane’s absence.